Journal: Frontiers in Toxicology

Article Title: Diesel exhaust particles alter mitochondrial bioenergetics and cAMP producing capacity in human bronchial epithelial cells

doi: 10.3389/ftox.2024.1412864

Figure Lengend Snippet: Exposure of BEAS-2B cells to DEP induced oxidative stress markers and differentially affected expression of key players of the antioxidant response system.

Article Snippet: BEAS-2B cells were exposed to 100 or 300 μg/mL DEP (SRM 2975, National Institute of Standards and Technology) for 24 h. Culture medium was collected to measure IL-8 and IL-6 protein concentrations using specific enzyme-linked immunosorbent assays (ELISAs) ( ).

Techniques: Expressing, Control

Journal: Purinergic Signalling

Article Title: Autocrine signaling via release of ATP and activation of P2X7 receptor influences motile activity of human lung cancer cells

doi: 10.1007/s11302-014-9411-x

Figure Lengend Snippet: TGF-β1-induced migration is mediated by release of ATP and activation of P2X7 receptor in H292 lung cancer cells, but not BEAS-2B normal lung cells. a The expression of P2X7 receptor in BEAS-2B, A549, PC-9, and H292 cells was detected by immunoblotting as described in “Materials and methods”. b, c BEAS-2B cells (b) or H292 cells (c) were stimulated with TGF-β1 (5 ng/mL) and incubated for the indicated times, then the concentration of ATP in the culture medium was measured as described in “Materials and methods”. d, e Cell migration was examined by means of Transwell assay as described in “Materials and methods”. BEAS-2B cells (d) or H292 cells (e) were pretreated for 30 min with apyrase (20 U/mL) or A438079 (100 μM) and then stimulated for 24 h with TGF-β1 (5 ng/mL). The lower membrane surfaces were photographed through a microscope at ×20 magnification, and migrated cells in each field were counted. Values are means ± SE (n = 4–10). A significant difference between the indicated group and control group is indicated by *** or ### (P < 0.001), ** (P < 0.01), †(P < 0.05)

Article Snippet: Cell culture BEAS-2B normal human bronchial epithelial cell line and NCI-H292 human lung mucoepidermoid carcinoma cell line were purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Migration, Activation Assay, Expressing, Western Blot, Incubation, Concentration Assay, Transwell Assay, Microscopy